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Types of Analysis (Adhoc testing)

IMPORTANT INFORMATION

1.      SAGL’s prices can be revised without prior notice

2.      Submission of samples

2.1          Please ensure to include the time-line required for the forwarding of samples to SAGL, as well as the lead-time required by
               SAGL for the testing of samples, within your internal scheduling to prevent placing SAGL under any undue pressure and to 
               enable SAGL to present you with a reliable and accurate service.  

2.2          Sample masses must be determined according to the list of testing required

2.3          Each sample consignment must include an analyses request (contact information, order number, sample reference(s), list of
               analyses, different components for product mixtures (if applicable to your samples) and Regulatory information (if
               applicable to your testing). 

2.4          Sample consignments which do not include an analyses request will be placed on hold until customer/sample traceability
               has been established and pending the receival of an analyses request.
 
2.5          SAGL’s analyses request form is available for download on our website (www.sagl.co.za, Select “Services”, “Type of
               Analyses” and “Print Analyses Request Form”). 

2.6          It remains the customers’ responsibility to clearly mark each sample with the specific matrix information when submitting
               samples to the SAGL for testing, as SAGL will determine if products are in or out of scope of each methodology based on 
               this information during the registration process. 

2.7          All postage, railage, airfreight, courier and other charges for delivery to the SAGL must be paid by the consignor, unless
               agreed differently beforehand with the General Manager of the SAGL.  SAGL will only become responsible for the sample
               consignment after the samples have been received. 

 

3.      Packaging of samples

3.1          All samples must be packaged in such a way as to prevent damage during transit. 

3.2          Samples for moisture calibration purposes must be packaged in screw cap containers as environmental conditions can
               influence the sample’s moisture content.  Sample containers must be filled to the top. 

3.3          Samples for vitamin testing must be protected from sunlight and heat.

  

4.      General

4.1          Lead-times will be determined by the list of testing and number of samples received per consignment, as well as testing
               already scheduled within SAGL’s system at the time of receiving the sample consignment. 

4.2          Moisture content, protein content and mixogram is a factor in certain analyses, the fee for a single moisture, protein and/or
               mixogram analysis will be charged additionally per sample, where applicable. 

4.3          Should the product matrix not be listed under the methodologies scope, SAGL will document the following note on the test
               report:  Product does not form part of the method's scope.    

4.4          Granulation (Particle size):  Five sieves and the pan is included in the cost for one sieve test.  Customers are requested to
               list the sieve sizes on the analyses request according to their product application. List of SAGL sieves 

4.5          Preparation costs for samples/requests which fall outside our normal scope of testing, can only be accurately determined
               after the receival of samples. 

4.6          Vitamin and mineral testing:
               -Please forward any available vitamin and mineral specs with the sample consignment to assist SAGL with sample dilutions
                during the preparation of samples for vitamin analyses.

               -Please inform the SAGL should you submit samples for vitamin or mineral analysis that you suspect might fall outside the
                specs of the fortification regulations, as this will assist SAGL in the evaluation of the test results and prevent unnecessary
                repeat analysis for confirmation purposes. Please also note that additional invoicing will be applicable for additional
                dilutions as this will result in an additional analysis to obtain a test result. 

4.7          The test results of calibration samples cannot be guaranteed without homogeneity testing.

4.8          Test results relate only to the samples tested. 

4.9          Uncertainty of measurement values for specific analytical methods are available on request.  Uncertainties are calculated
               on a 95 % confidence level, coverage factor 1.96. 

4.10        If available, sealed arbitration samples are stored for one month by the SAGL for possible future enquiries.

 

4.11        Test report may not be reproduced, except in full.  When only certain pages or sections of the full report are reproduced,
               written permission must be given by the General Manager. 

4.12        The SAGL or its members shall in no way be liable for any error made when carrying out a test or erroneous statement,
               whether in fact or in opinion. 


5.      Financial

 

5.1          Please note that all prices are excluding VAT.

5.2          Samples received without an order number will be placed on hold until the order number has been received.

5.3          SAGL’s Policy for new clients require upfront payment for test reports during the first 6 months and thereafter on a 30 day
               account, subject to the completed documents received. Terms are strictly 30 days and must be paid on the receipt of
               statement. 

5.4          Foreign customers must settle their account and the payment has to reflect on our banking account before report can be
               forwarded. 

5.5          Preliminary reports will be invoiced at an additional fee.

 

5.6          Single-Sample-Price Principal:  The single-sample-price applies when a customer submits only one sample for certain 
               analyses. 

5.7          Sliding scale prices will be implemented where applicable on analyses/projects.

    

Print Analyse request form- click here


Wheat Grading

Analysis particulars

Full wheat grading without cultivar identification / (2.5kg sample)

Hectolitre mass (Kern 222: minimum mass 1.5kg)  (Method SANAS Accredited)         

Ergot (2.5kg sample), %

Foreign matter, %

*Heat damaged kernels, %              

*Immature kernels, %                                                                       

*Insect damaged kernels, %            

*Sprouted kernels, %         

*Total Damaged kernels

Heavily frost damaged kernels, %

Fungi (field/storage), %                                   

Noxious seeds (10kg sample required), Total / 10kg

Other grain and unthreshed ears, %             

Screenings, % (1.8mm) (Method SANAS Accredited)                                                         

Undesirable odours                                                         

 Hectolitre mass is carried out according to ISO 7971-3,2009 (E). Remaining wheat grading is carried out according to Government Gazette grading regulations
(Minimum mass: 2.5kg)


Maize Grading

Analysis particulars

Full maize grading

Defective kernels (Method SANAS Accredited)

Lemon Yellow, %

Full maize grading is carried out according to the Government Gazette grading regulations
(minimum mass: 2kg)

 

Other Grading

 

Analysis particulars

Grading of sunflower (minimum mass: 5kg)
Grading of Soya (minimum mass: 10kg)

 

Physical Tests

Analysis particulars

Reference

100 Kernel mass (maize), g

Industry Accepted Method 001

1000 Kernel mass (wheat), g

Industry Accepted Method 008

Breakage susceptibility,  g < 6.35mm, g < 4.75mm

Industry Accepted Method 007

Kernel size (10mm, 8mm, 6.35mm, 4.8mm, 2.4mm) (Maize), %

Industry Accepted Method 017

Pap cooking test

Industry Accepted Method 005

Granulation/Particle size (Wheat flour), % (Please note the applicable information listed under General)

Industry Accepted Method 003

Granulation/Particle size (Maize products), % (Please note the applicable information listed under General)

Industry Accepted Method 003

Screenings  (Wheat) (USA), % (1.6mm)

U.S. Department of Agriculture, GIPSA, Federal Grain Inspection Handbook II, Wheat, 6/1/97

Sieve test (Maize) (USA), % (4.8mm, 2.4mm)

U.S. Department of Agriculture, GIPSA, Federal Grain Inspection Handbook II, Corn, 6/1/97

Speck count , 100cm2 (minimum mass: 1kg)     

Industry Accepted Method 002

Stress cracks, %

Industry Accepted Method 006

 

Milling

Analysis particulars

Reference

Bühler  Extraction (2‑6kg)

Industry Accepted Method 011

Cleaning of wheat on the Dockage Tester

Before milling

Vitreous kernels, %

Industry Accepted Method 009

Quadromat milling (Durum)

Industry Accepted Method 024

Quadromat milling (Wheat)

Industry Accepted Method 024

Roff 150 (Maize)

Industry Accepted Method 013

 

Chemical Analysis

Analysis particulars

Reference

Ash, % (Moisture is a factor)

In-house Method 011 (Method SANAS Accredited)

Bran content, % (212 µm)

Government Notice R. 186 of 22 February 2008

Colour, Kent Jones (KJ)   

FTP Method No. 0007/3, 7/1991

CALCULATIONS

 

Carbohydrates (by difference), g/100g (Calculated value)

SOP MC23 (SOP SANAS Accredited)(The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Baking worth

Analyses required for calculation:  Moisture, protein, Farinogram, Alveogram, Mixogram, 100g baking test

Energy value (metabolizable), kJ/100g (Calculated value)

SOP MC23 (SOP SANAS Accredited) (The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Total digestible nutritional value, % 

(Calculated value)

SOP MC23 (SOP SANAS Accredited) (The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Milling Worth

Analyses required for calculation:  Hectolitre mass, 1000 kernel mass, Büler milling, KJ colour, moisture, Falling Number

Additional calculations on request (E.g.:  as is, dry basis, 12 % moisture basis)

Damaged starch, % (Moisture is a factor)     

In-house Method 23

Diastatic activity, mg/10g (Maltose)

In-house Method 25

Air drying and milling of bread

Dry solids determination of bread

Government Notice R. 186 of 22 February 2008

Falling Number, sek.  (Moisture is a factor / minimum mass: 300g)

ICC Std No. 107/1, Latest Edition (Method SANAS Accredited)

Fat Crude (Petroleum ether extraction) (Soxhlet) , %  (Moisture is a factor)

In-house Method 024 (Method SANAS Accredited)

Fibre Crude (Acid–base digestion – 3 days), % (Moisture is a factor) 

In-house Method 20

Fibre Dietary (total), % (g/100) (Fat content is a factor)

In-house Method 012 (Method SANAS Accredited)

GMO (Bt-Cry1Ab – maize and maize meal)

SDI Bt maize Kit User's Guide, Rev. 011601, Ver. 1.4 (ELISA)

GMO (RUR-NK603 – maize and maize meal)

SDI Soya Test Kit with RUR NK603 Maize Testing Protocol(ELISA)

GMO (RUR-CP4 – Soya beans and ground Soya beans)

SDI Soya Test Kit User's Guide, Rev. 031703, Ver. 2.1 (ELISA)

Hunterlab colour, Lab

Industry Accepted Method 004

Milling index (Maize), Infratec (NIT)

Industry Accepted Method 015

Moisture (Barley – 2 hour oven milled kernels), % (130 – 133 °C)

Analytica EBC Method 3.2, Latest Edition (Method SANAS Accredited)

Moisture (Canola – 17 hour oven whole kernels), % (103 °C)

ISTA, Section 9, Latest Edition

Moisture (milled Maize – 1 hour oven), %(130 °C)               

AACC 44-15.02, Latest Edition (Method SANAS Accredited)

Moisture (milled Soya beans – 17 hour oven), %(103 °C)               

ISTA Handbook, Section 9, Latest Edition

Moisture (Maize/Soya beans – 72 hour oven whole kernels / beans,  % (103 oC)

AACC 44-15.02, Latest Edition (Method SANAS Accredited)

Moisture (Malt – 3 hour oven milled kernels), % (106 °C)

Analytica EBC Method 4.1, Latest Edition

Moisture (Sorghum – 2 hours oven milled kernels), % (130 °C)

ISTA, Section 9, Latest Edition

Moisture (Sorghum – 72 hours oven whole kernels), % (103 °C)

Sorghum Grading Regulations No. R.573 (Paragraph 19) (AACC 45-15.02)

Moisture (Sunflower – 3+ hour oven whole seeds), % (103°C)

ISO 665 – 1977 E

Moisture (Wheat – 72 hour oven whole kernels), % (103 oC)

Government Gazette No 33860 dated 17 December 2010, Regulation No.R.1186 (Method SANAS Accredited)

Moisture (Wheat/Rice/Millet milled kernels – 2 hour oven), % (130 °C)          

ICC Std No. 110/1, Latest Edition (Method SANAS Accredited)

Moisture (Wheat flour – 90 minutes oven), % (130 °C)         

ICC Std No. 110/1, Latest Edition (Method SANAS Accredited)

Moisture (Vegetable seeds), %

ISTA Section 9, Latest Edition (Whole seeds or milling, oven duration and temperature differs between species)

pH

AACC 02-52.01, Latest Edition

Protein (crude) – other, % Dumas (Leco) (Moisture is a factor) 
Protein (crude) – on wet samples

AACC 46-30.01, Latest Edition (Method SANAS Accredited)

Starch content, % (Polarimeter) (Moisture is a factor)

In-house Method 019

Whiteness index  87:13 sifted (300µm)

Industry Accepted Method 004

Whiteness index unsifted

Industry Accepted Method 004

Whiteness index unsifted & sifted

Industry Accepted Method 004

 

Mycotoxins

Analysis particulars

Reference

Determining the Multi-Mycotoxins using  UPLC-MS/MS: Includes Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Fumonisin B1, T2-Toxin, Deoxynivalenol, Ochratoxin A and Zearalenone
Reporting of results: ppb = µg/kg

In-house Method 026 (UPLC-MS/MS) (Method SANAS Accredited)

Aflatoxin (total) ppb/dpb = µg/kg

ROSA Quantitative Method, Latest Edition

Deoxynivalenol (DON) ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

Fumonisin ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

Ochratoxin A, ppb/dpb = µg/kg

ROSA Quantitative Method, Latest Edition

Zearalenone ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

 

HPLC and AA

Analysis particulars

Reference

AMINO ACIDS – Sliding scale prices will be implemented where applicable on analysis/projects

Protein bound amino acids (Foodstuffs)

In-house Method 009 (HPLC)

Protein bound amino acids (Pharmaceutical)

In-house Method 009 (HPLC)

Tryptophan

In-house Method 007 (HPLC)

Cysteine and/or Methionine

In-house Method 015 (HPLC)

Melamine  – Sliding scale prices will be applicable on more than 1 sample received simultaneously

HPLC determination (positive results must be verified using mass spectrometry)

In-house method IH 022 (HPLC)

Confirmation of positive results: Samples will be subcontracted

Please note the following: 
-  The verification of positive test results will be at an additional fee and additional lead-time
- The lead-time will be determined by the subcontracted laboratory.  Should customers request that SAGL negotiate a shorter lead-time with the subcontracted laboratory, an urgency rate may be applicable.

SUDAN RED

Package for Sudan I, II, III and IV/Para Red

Minimum 10 samples per analyses request

In-house Method 005 (HPLC)

VITAMINS

Ascorbic Acid (Vitamin C)

 In-house Method 016 (HPLC)

Folic Acid (Vitamin B9) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 003 (HPLC) (Method SANAS Accredited)

Nicotinamide (Moisture is a factor when testing according to the fortification regulation)

In-house Method 002 (HPLC) (Method SANAS Accredited)

Pyridoxine (Vitamin B6) (Moisture is a factor when testing according to the fortification regulation) 

In-house Method 002 (HPLC) (Method SANAS Accredited)

Riboflavin (Vitamin B2) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 002 (HPLC) (Method SANAS Accredited)

Thiamine (Vitamin B1) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 002 (HPLC) (Method SANAS Accredited)

Vitamin A (Retinol saphonication) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 001 (HPLC) (Method SANAS Accredited)

Package for Fortification (Vitamins A, B1, B2, B6, B9, Nicotinamide) (Moisture is a factor when testing according to the fortification regulation) 

Vitamins:  HPLC In-house Method 001 – 003

MINERALS

Iron – Qualitative Method (Yes/No)

AACC 40-40.01, Latest Edition

Iron (Total) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 010 (AA) (Method SANAS Accredited)

Zinc (Total) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 010 (AA) (Method SANAS Accredited)

Regulations relating to the fortification of certain foodstuffs, 7 April 2003.  Government Gazette No. 24715 –(Wheat flour: 14 % moisture basis / Maize meal: 12.5% moisture basis / Wheat bread: 39% moisture basis)

 Rheological and Baking

Analysis particulars

Reference

Alveogram (Moisture is a factor)      

ICC Std No. 121, Latest Edition (Method SANAS Accredited)

Alveogram Extended Method (Moisture is a factor)                       

Application Notes for the Chopin Alveograph, Note No. 8, p.21

Baking test, (100 g) (Moisture, protein content and mixogram is a factor)   

Industry Accepted Method 022

Baking test (commercial) with or without drop test (the drop test will be included, unless otherwise requested)

Industry Accepted Method 018

Consistogram (Moisture is a factor)  

AACC 54-50.01, Latest Edition

Extensigram 45 minutes 
(Moisture is a factor)               

ICC Std No. 114/1, Latest Edition

Extensigram additional 90 minutes 90 minute       

ICC Std No. 114/1, Latest Edition

Farinogram (Moisture is a factor)   

AACC 54-21.01, Latest Edition – Constant Flour Weight Procedure (Method SANAS Accredited)

Gluten (flour – wet and/or dry) (Moisture is a factor)  

AACC 38 – 12.02, Latest Edition

Gluten Index (Moisture is a factor)  

AACC 38 – 12.02, Latest Edition

Mixogram (flour or whole wheat flour) (Moisture and protein content is a factor)  

Industry Accepted Method 020 (Method SANAS Accredited)